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Product Name
PPP3R1 antibody
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Description
PPP3R1 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive WB detected in K-562 cells, HeLa cells, human stomach tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 19 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Calcineurin subunit B type 1 antibody; CALNB1 antibody; CNA2 antibody; CNB antibody; CNB1 antibody; PPP3R1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPP3R1 recombinant protein (Accession Number: NM_000945). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:114114(PPP3R1 antibody) at dilution of 1:400
IP Result of anti-PPP3R1 (IP:Catalog No:114114, 3ug; Detection:Catalog No:114114 1:300) with HeLa cells lysate 5000ug.
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:114114(PPP3R1 antibody) at dilution of 1:100 (under 10x lens)
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Background
Protein phosphatase 3 (PPP3, formerly PP2B, Calcineurin), a serine/ threonine protein phosphatase, is a heterodimer composed of one catalytic subunit (PPP3C, Calcineurin A) and one regulatory subunit (PPP3R, Calcineurin B). PPP3R has two isoforms, PPP3R1 and PPP3R2. PPP3Rl is ubiquitously expressed in different tissues and is coded by the PPP3R1 gene which was mapped recently to human chromosome 2. PPP3Rl has four functional calcium-binding sites.
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References
- Su Z, Xin S, Xu L. The calcineurin B subunit induces TNF-related apoptosis-inducing ligand (TRAIL) expression via CD11b-NF-κB pathway in RAW264.7 macrophages. Biochemical and biophysical research communications. 417(2):777-83. 2012.
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