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Product Name
PPP2R5C Polyclonal Antibody
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Description
Polyclonal antibody to PPP2R5C
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
PPP2R5C antibody; B56G antibody; PR61G antibody; B56gamma antibody; protein phosphatase 2 regulatory subunit B'gamma antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human PPP2R5C (NP_848702.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - PPP2R5C Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PPP2R5C antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - PPP2R5C Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using PPP2R5C antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PPP2R5C Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using PPP2R5C antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PPP2R5C Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using PPP2R5C antibody at dilution of 1:100 (40x lens).
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Background
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"