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Product Name
PPP2R2C antibody
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Description
PPP2R2C Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, MCF7 cells, mouse testis tissue. Positive IHC detected in human breast cancer tissue, human brain tissue, human testis tissue. Positive FC detected in MCF-7 cells. Observed molecular weight by Western-blot: 49kd
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Tested applications
ELISA, IHC, WB, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B55 GAMMA antibody; IMYPNO antibody; IMYPNO1 antibody; PP2A subunit B isoform gamma antibody; PPP2R2C antibody; PR52 antibody; PR55G antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPP2R2C recombinant protein (Accession Number: NM_001206996). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114101(PPP2R2C antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114101(PPP2R2C antibody) at dilution of 1:50 (under 40x lens)
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114101(PPP2R2C Antibody) at dilution of 1:1000
1X10^6 MCF-7 cells were stained with 0.2ug PPP2R2C antibody (Catalog No:114101, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
PPP2R2C represents protein phosphatase 2 (formerly 2A), regulatory subunit B, gamma isoform
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References
- Chen L, Lai Y, Zhu X. The role of specific PP2A complexes in the dephosphorylation of γH2AX. Journal of cell science. 128(2):421. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"