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Product Name
PPP2R2A Polyclonal Antibody
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Description
Polyclonal antibody to PPP2R2A
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
PPP2R2A antibody; B55A antibody; B55ALPHA antibody; PR52A antibody; PR55A antibody; PR55alpha antibody; protein phosphatase 2 regulatory subunit Balpha antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-447 of human PPP2R2A (NP_002708.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - PPP2R2A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PPP2R2A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - PPP2R2A Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using PPP2R2A antibody . Blue: DAPI for nuclear staining.
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Background
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"