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Product Name
PPP1R14A Polyclonal Antibody
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Description
Polyclonal antibody to PPP1R14A
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
PPP1R14A antibody; CPI-17 antibody; CPI17 antibody; PPP1INL antibody; protein phosphatase 1 regulatory subunit 14A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human PPP1R14A (NP_150281.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - PPP1R14A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PPP1R14A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 60s.
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Background
Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"