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Product Name
PPP1CB-Specific antibody
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Description
PPP1CB-Specific Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, NIH/3T3. Positive IHC detected in human prostate cancer tissue. Positive IF detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 37 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PP1 Beta antibody; PP 1B antibody; PPP1CB antibody; PPP1CD antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPP1CB-Specific recombinant protein (Accession Number: NM_002709). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114143(PPP1CB antibody) at dilution of 1:1500
NIH3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:114143(PPP1CB antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114143(PPP1CB antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-PPP1CB (IP:Catalog No:114143, 4ug; Detection:Catalog No:114143 1:1000) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed NIH/3T3 cells using Catalog No:114143(PPP1CB Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Protein phosphatase 1 (PP1) is the main eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular processes including protein-protein interactions, gene transcription, cell-cycle progression and apoptosis. It is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. The catalytic subunit of PP1 (PP1c) can interact with amount of regulatory subunits in vivo that target it to particular subcellular locations and regulate its activity towards specific substrates. PPP1CB migrated as a 37kd protein on SDS page. This antibody is specific to PPP1CB.
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References
- Sun D, Zhou M, Kowolik CM. Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma. Melanoma research. 21(4):335-43. 2011.
- Huang C, Cao W, Liao R. PP1γ functionally augments the alternative splicing of CaMKIIδ through interaction with ASF. American journal of physiology. Cell physiology. 306(2):C167-77. 2014.
- Liao RJ, Tong LJ, Huang C. Rescue of cardiac failing and remodelling by inhibition of protein phosphatase 1γ is associated with suppression of the alternative splicing factor-mediated splicing of Ca2+/calmodulin-dependent protein kinase δ. Clinical and experimental pharmacology & physiology. 41(12):976-85. 2014.
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