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Product Name
PPL Polyclonal Antibody
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Description
Polyclonal antibody to PPL
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PPL antibody; periplakin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PPL (NP_002696.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - PPL Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PPL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunofluorescence - PPL Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using PPL antibody . Blue: DAPI for nuclear staining.
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Background
Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"