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Product Name
PPIH antibody
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Description
PPIH Rabbit Polyclonal antibody. Positive WB detected in PC-3 cells, human colon tissue. Positive IHC detected in human colon cancer tissue. Positive IF detected in PC-3 cells. Observed molecular weight by Western-blot: 20kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CYP 20 antibody; CYP20 antibody; CYPH antibody; PPIase H antibody; PPIH antibody; Rotamase H antibody; SnuCyp 20 antibody; USA CYP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPIH recombinant protein (Accession Number: NM_006347). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:114126(PPIH antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114126(PPIH antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of PC-3 cells using Catalog No:114126(PPIH Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PPIH(Peptidyl-prolyl cis-trans isomerase H) is also named as CYP20, CYPH and belongs to cyclophilin-type PPIase family, PPIase H subfamily.It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may act as a chaperone(PMID:12875835).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"