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Product Name
PPID antibody
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Description
PPID Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human cervical cancer tissue. Positive WB detected in mouse brain tissue, A549 cells, human brain tissue, Jurkat cells, MCF7 cells, NIH/3T3 cells, Raji cells, rat brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 38-41 kDa
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Tested applications
ELISA, WB, IHC, FC, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
Cyclophilin 40 antibody; Cyclophilin D antibody; Cyclophilin related protein antibody; CYP 40 antibody; CYP40 antibody; CYPD antibody; peptidylprolyl isomerase D antibody; PPIase D antibody; PPID antibody; Rotamase D antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPID recombinant protein (Accession Number: NM_005038). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114123(PPID antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:114123(PPID antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:114123(PPID antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-PPID (IP:Catalog No:114123, 4ug; Detection:Catalog No:114123 1:1000) with mouse brain tissue lysate 4000ug.
1X10^6 HeLa cells were stained with 0.2ug PPID antibody (Catalog No:114123, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
PPID(Peptidyl-prolyl cis-trans isomerase D) is also named as CYP40, CYPD and belongs to the cyclophilin-type PPIase family,which accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339).
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