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Product Name
PPFIBP1 antibody
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Description
PPFIBP1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Positive WB detected in HepG2 cells, DU 145 cells. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 114 kDa
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Tested applications
ELISA, WB, IHC, IP, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hSGT2 antibody; hSgt2p antibody; KIAA1230 antibody; Liprin beta 1 antibody; PPFIBP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPFIBP1 recombinant protein (Accession Number: NM_177444). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114121(PPFIBP1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114121(PPFIBP1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114121(PPFIBP1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-PPFIBP1 (IP:Catalog No:114121, 4ug; Detection:Catalog No:114121 1:500) with HepG2 cells lysate 2400ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:114121(PPFIBP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HepG2 cells were stained with .2ug PPFIBP1 antibody (Catalog No:114121, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
PPFIBP1 represents PTPRF interacting protein, binding protein 1 (liprin beta 1)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"