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Product Name
PPFIA3 antibody
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Description
PPFIA3 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, HEK-293 cells. Observed molecular weight by Western-blot: 140 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA0654 antibody; Liprin alpha 3 antibody; LPNA3 antibody; PPFIA3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPFIA3 recombinant protein (Accession Number: BC021255). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:114120(PPFIA3 Antibody) at dilution of 1:600
IP Result of anti-PPFIA3 (IP:Catalog No:114120, 4ug; Detection:Catalog No:114120 1:600) with mouse brain tissue lysate 3440ug.
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Background
PPFIA3, also named as KIAA0654, belongs to the liprin family and Liprin-alpha subfamily. PPFIA3 may regulate the disassembly of focal adhesions. It may localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates. PPFIA3 exhibites more than 5 fold accumulations in the monoribosome fraction. PPFIA3 is decreased at the protein level by the miR-99 family but not in the luciferase reporter with the 3’UTR of PPFIA3. (PMID:21212412)
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References
- Sun D, Lee YS, Malhotra A. miR-99 family of MicroRNAs suppresses the expression of prostate-specific antigen and prostate cancer cell proliferation. Cancer research. 71(4):1313-24. 2011.
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