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Product Name
PPAT antibody
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Description
PPAT Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in human stomach tissue, HEK-293 cells, HepG2 cells. Observed molecular weight by Western-blot: 50-56kd
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Amidophosphoribosyltransferase antibody; ATASE antibody; GPAT antibody; PPAT antibody; PRAT antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPAT recombinant protein (Accession Number: NM_002703). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
human stomach tissue were subjected to SDS PAGE followed by western blot with Catalog No:114086(PPAT antibody) at dilution of 1:500
IP Result of anti-PPAT (IP:Catalog No:114086, 4ug; Detection:Catalog No:114086 1:500) with HepG2 cells lysate 2400ug.
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Background
PPAT represents phosphoribosyl pyrophosphate amidotransferase
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References
- Sugiyama K, Ebinuma H, Nakamoto N. Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus. PloS one. 9(4):e94460. 2014.
- Mitsuishi Y, Taguchi K, Kawatani Y. Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming. Cancer cell. 22(1):66-79. 2012.
- Dong D, Qi Y, Xu L. Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats. Food & function. 5(12):3065-75. 2014.
- Kaneko YS, Takayanagi T, Nagasaki H. Aripiprazole increases NAD(P)H-quinone oxidoreductase-1 and heme oxygenase-1 in PC12 cells. Journal of neural transmission (Vienna, Austria : 1996). 122(6):757-72. 2015.
- Liu M, Xu L, Yin L. Potent effects of dioscin against obesity in mice. Scientific reports. 5:7973. 2015.
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