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Product Name
PPAR alpha Monoclonal Antibody
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Description
Monoclonal antibody to PPAR alpha
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Tested applications
WB, IHC, IF, FC
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Species reactivity
Human, Mouse
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Alternative names
PPARA antibody; NR1C1 antibody; PPAR antibody; PPARalpha antibody; hPPAR antibody; peroxisome proliferator-activated receptor alpha antibody
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Isotype
Mouse IgG
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Preparation
Antigen: Recombinant protein of human PPAR alpha
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:10 - 1:25
IF 1:10 - 1:25
FC 1:10 - 1:25 -
Validations
Western blot - PPAR alpha Monoclonal Antibody
Western blot analysis of extracts of various cell lines, using PPAR alpha antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"