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Product Name
PPA1 antibody
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Description
PPA1 Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, human liver tissue. Positive IHC detected in human breast cancer tissue, human heart tissue. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Inorganic pyrophosphatase antibody; IOPPP antibody; PP1 antibody; PPA1 antibody; PPase antibody; pyrophosphatase (inorganic) 1 antibody; SID6 8061 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPA1 recombinant protein (Accession Number: NM_021129). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:114077(PPA1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114077(PPA1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114077(PPA1 antibody) at dilution of 1:50 (under 40x lens)
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Background
PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).
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