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Product Name
POU6F1 Polyclonal Antibody
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Description
Polyclonal antibody to POU6F1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
POU6F1 antibody; BRN5 antibody; MPOU antibody; TCFB1 antibody; POU class 6 homeobox 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human POU6F1 (NP_002693.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - POU6F1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using POU6F1 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunofluorescence - POU6F1 Polyclonal Antibody
Immunofluorescence analysis of A-549 cells using POU6F1 antibody .
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Background
Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3').
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"