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Product Name
POU5F1B Polyclonal Antibody
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Description
Polyclonal antibody to POU5F1B
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Tested applications
WB
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Species reactivity
Human
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Alternative names
POU5F1B antibody; OCT4-PG1 antibody; OCT4PG1 antibody; OTF3C antibody; OTF3P1 antibody; POU5F1P1 antibody; POU5F1P4 antibody; POU5FLC20 antibody; POU5FLC8 antibody; POU class 5 homeobox 1B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human POU5F1B (NP_001153014.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - POU5F1B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using POU5F1B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Shows weak transcriptional activator activity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"