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Product Name
POU3F3-Specific antibody
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Description
POU3F3-Specific Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive WB detected in SH-SY5Y cells, HEK-293 cells, human brain tissue, mouse brain tissue. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Brain 1 antibody; Brn 1 antibody; BRN1 antibody; OCt 8 antibody; OCtamer binding protein 8 antibody; OTF 8 antibody; OTF8 antibody; POU class 3 homeobox 3 antibody; POU3F3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_006236). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:114071(POU3F3-Specific antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:114071(POU3F3-Specific antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:114071(POU3F3-Specific antibody) at dilution of 1:100 (under 40x lens)
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Background
OTF8, also named as POU3F3 and BRN1, belongs to the POU transcription factor family and Class-3 subfamily. The antibody is specific to OTF8.
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