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Product Name
POU2F3 Polyclonal Antibody
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Description
Polyclonal antibody to POU2F3
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Tested applications
WB
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Species reactivity
Mouse
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Alternative names
POU2F3 antibody; Epoc-1 antibody; OCT-11 antibody; OCT11 antibody; OTF-11 antibody; PLA-1 antibody; PLA1 antibody; Skn-1a antibody; POU class 2 homeobox 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human POU2F3 (NP_055167.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - POU2F3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using POU2F3 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
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Background
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Regulated the expression of a number of genes such as SPRR2A or placental lactogen.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"