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Product Name
POU2AF1 Polyclonal Antibody
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Description
Polyclonal antibody to POU2AF1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
POU2AF1 antibody; BOB1 antibody; OBF-1 antibody; OBF1 antibody; OCAB antibody; POU class 2 associating factor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human POU2AF1 (NP_006226.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - POU2AF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using POU2AF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - POU2AF1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using POU2AF1 antibody . Blue: DAPI for nuclear staining.
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Background
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"