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Product Name
POT1 antibody
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Description
POT1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, PC-3 cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 71 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DKFZp586D211 antibody; hPot1 antibody; POT1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of POT1 recombinant protein (Accession Number: XM_006715917). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114065(POT1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114065(POT1 antibody) at dilution of 1:100 (under 25x lens)
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Background
Protection of telomeres 1(POT1)is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase.
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References
- Singh U, Maturi V, Jones RE, Paulsson Y, Baird DM, Westermark B. CGGBP1 phosphorylation constitutes a telomere-protection signal. Cell cycle (Georgetown, Tex.). 13(1):96-105. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"