Poneratoxin peptide

Poneratoxin peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

FLPLLILGSLLMTPPVIQAIHDAQR-NH2

Phe-Leu-Pro-Leu-Leu-Ile-Leu-Gly-Ser-Leu-Leu-Met-Thr-Pro-Pro-Val-Ile-Gln-Ala-Ile-His-Asp-Ala-Gln-Arg-NH2

25

97.59%

C₁₂₉H₂₁₅N₃₃O₃₁S₁

2756.3

Synthetic

Powder

Poneratoxin is a paralyzing neurotoxic peptide made by the bullet ant Paraponera clavata. It prevents inactivation of voltage gated sodium channels and therefore blocks the synaptic transmission in the central nervous system. Specifically, poneratoxin acts on voltage gated sodium channels in skeletal muscle fibers, causing paralysis, and nociceptive fibers, causing pain. It is rated as a 4 plus on the Schmidt sting pain index, the highest in the world, and its effects can cause waves of pain up to twelve hours after a single sting.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Szolajska E, Poznanski J, Ferber ML, et al. Poneratoxin, a neurotoxin from ant venom. Structure and expression in insect cells and construction of a bio-insecticide. Eur J Biochem. 2004;271(11):2127-36
• Robinson SD, Deuis JR, Touchard A, Keramidas A, Mueller A, Schroeder CI, Barassé V, Walker AA, Brinkwirth N, Jami S, Bonnafé E, Treilhou M, Undheim EAB, Schmidt JO, King GF, Vetter I. Ant venoms contain vertebrate-selective pain-causing sodium channel toxins. Nat Commun. 2023 May 23;14(1):2977. doi: 10.1038/s41467-023-38839-1. PMID: 37221205; PMCID: PMC10206162.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.