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Product Name
PON1 antibody
- Documents
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Description
PON1 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive WB detected in mouse liver tissue, human blood tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 42-45 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
A esterase 1 antibody; Aromatic esterase 1 antibody; ESA antibody; K 45 antibody; paraoxonase 1 antibody; PON antibody; PON 1 antibody; PON1 antibody; Serum aryldialkylphosphatase 1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PON1 recombinant protein (Accession Number: NM_000446). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:114058(PON1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:114058(PON1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:114058(PON1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-PON1 (IP:Catalog No:114058, 4ug; Detection:Catalog No:114058 1:500) with mouse liver tissue lysate 4000ug.
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Background
PON1, also named as PON and K-45, belongs to the paraoxonase family. PON1 hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It can capable of hydrolyzing a broad spectrum of organophosphate substrates and a number of aromatic carboxylic acid esters. PON1 may mediate an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. For glycosylated, the MW of PON1 is migrated 42-45kd. 55kd is a dimmer of isoform CRA-b. The antibody has cross-reaction to PON2 and PON3.
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