-
Product Name
POMGNT1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to POMGNT1
-
Tested applications
WB, IHC
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
POMGNT1 antibody; GNTI.2 antibody; GnT I.2 antibody; LGMD2O antibody; MEB antibody; MGAT1.2 antibody; RP76 antibody; gnT-I.2 antibody; GnTI.2 antibody; protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 411-660 of human POMGNT1 (NP_060209.3).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - POMGNT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using POMGNT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunohistochemistry - POMGNT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using POMGNT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - POMGNT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using POMGNT1 antibody at dilution of 1:100 (40x lens).
-
Background
Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose and does not have MGAT3, MGAT4, MGAT5, MGAT7 or MGAT8 activity.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"