-
Product Name
POM121 antibody
- Documents
-
Description
POM121 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human cervical cancer tissue, human colon tissue, rat colon tissue. Observed molecular weight by Western-blot: 140-150 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human, Rat; other species not tested.
-
Alternative names
DKFZp586G1822 antibody; DKFZp586P2220 antibody; FLJ41820 antibody; KIAA0618 antibody; Nucleoporin Nup121 antibody; NUP121 antibody; POM121 antibody; POM121A antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of POM121 recombinant protein (Accession Number: NM_001387685). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114056(POM121 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded rat colon tissue slide using Catalog No:114056(POM121 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:114056(POM121 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:114056(POM121 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
POM121 is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Catalog#15645-1-AP do well in identifying the nuclear envelope in immunofluorescence experiments.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"