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Product Name
POLR3K antibody
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Description
POLR3K Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IF detected in HepG2 cells. Positive WB detected in Transfected HEK-293 cells.
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C11 antibody; C11 RNP3 antibody; hRPC11 antibody; HsC11p antibody; My010 antibody; POLR3K antibody; RNA polymerase III subunit C10 antibody; RNA polymerase III subunit C11 antibody; RPC10 antibody; RPC11 antibody; RPC12.5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of POLR3K recombinant protein (Accession Number: NM_016310). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:114054(POLR3K antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using POLR3K antibody Catalog No:114054 at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114054(POLR3K Antibody) at dilution of 1:1000
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Background
POLR3K represents polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"