-
Product Name
POLR2C Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to POLR2C
-
Tested applications
WB, IP
-
Species reactivity
Human, Mouse
-
Alternative names
POLR2C antibody; RPB3 antibody; RPB31 antibody; hRPB33 antibody; hsRPB3 antibody; RNA polymerase II subunit C antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human POLR2C (NP_116558.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IP 1:20 - 1:50 -
Validations
Western blot - POLR2C Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using POLR2C antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation - POLR2C Polyclonal Antibody
Immunoprecipitation analysis of 150ug extracts of SW620 cells using 3ug POLR2C antibody . Western blot was performed from the immunoprecipitate using POLR2C antibody at a dilition of 1:500.
-
Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"