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Product Name
POLR2B Polyclonal Antibody
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Description
Polyclonal antibody to POLR2B
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
POLR2B antibody; POL2RB antibody; RPB2 antibody; hRPB140 antibody; RNA polymerase II subunit B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - POLR2B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using POLR2B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - POLR2B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using POLR2B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - POLR2B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using POLR2B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - POLR2B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using POLR2B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - POLR2B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using POLR2B antibody at dilution of 1:100 (40x lens).
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Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"