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Product Name
POLR1D Polyclonal Antibody
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Description
Polyclonal antibody to POLR1D
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
POLR1D antibody; AC19 antibody; POLR1C antibody; RPA16 antibody; RPA9 antibody; RPAC2 antibody; RPC16 antibody; RPO1-3 antibody; TCS2 antibody; RNA polymerase I subunit D antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human POLR1D (NP_057056.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - POLR1D Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using POLR1D antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 40s.
Immunofluorescence - POLR1D Polyclonal Antibody
Immunofluorescence analysis of A549 cells using POLR1D antibody . Blue: DAPI for nuclear staining.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"