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Product Name
POLR1A antibody
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Description
POLR1A Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 190-195 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
A190 antibody; DKFZp586M0122 antibody; FLJ21915 antibody; POLR1A antibody; RNA polymerase I subunit A1 antibody; RPA1 antibody; RPA194 antibody; RPO1 4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of POLR1A recombinant protein (Accession Number: BC017115). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114034(RPA1 antibody) at dilution of 1:1000
IP Result of anti-RPA1 (IP:Catalog No:114034, 4ug; Detection:Catalog No:114034 1:300) with HeLa cells lysate 2400ug.
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Background
POLR1A, DNA-directed RNA polymerase I subunit RPA1, is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. RNA polymerase I (Pol I) located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I ranscription. Together with the second largest subunit, it is the catalytic core component of RNA polymerase I that synthesizes ribosomal RNA precursors.
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