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Product Name
POLI antibody
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Description
POLI Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue, human heart tissue. Positive IP detected in mouse testis tissue. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 87 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DNA polymerase iota antibody; Eta2 antibody; POLI antibody; polymerase (DNA directed) iota antibody; RAD30 homolog B antibody; RAD30B antibody; RAD3OB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of POLI recombinant protein (Accession Number: NM_001351632). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:114003(POLI antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114003(POLI antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114003(POLI antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-POLI (IP:Catalog No:114003, 4ug; Detection:Catalog No:114003 1:1000) with mouse testis tissue lysate 4000ug.
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Background
Mammalian Pol ι owns an unusual combination of properties: it is stimulated by Mn2+ ions, can bypass some DNA lesions and misincorporates “G” opposite template “T” more frequently than incorporates the correct “A.” [PMID:21304950] It also was shown to possess dRP lyase activity. In addition, Pol ι is much more efficient in the presence of Mn2+ in comparison to Mg2+. [PMID: 17609217]. It has been proposed that POLI involves in immunoglobulin somatic hypermutation (SHM), bypass of deaminated cytosines, several adducts of the purine bases. DNA strand crosslinks and is involved in DNA repair under oxidative stress. [PMID:15199127]
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References
- Makarova IV, Kazakov AA, Makarova AV. Transient expression and activity of human DNA polymerase iota in loach embryos. Biotechnology letters. 34(2):205-12. 2012.
- Sun H, Zou S, Zhang S. Elevated DNA polymerase iota (Poli) is involved in the acquisition of aggressive phenotypes of human esophageal squamous cell cancer. International journal of clinical and experimental pathology. 8(4):3591-601. 2015.
- Iguchi M, Osanai M, Hayashi Y, Koentgen F, Lee GH. The error-prone DNA polymerase ι provides quantitative resistance to lung tumorigenesis and mutagenesis in mice. Oncogene. 33(27):3612-7. 2014.
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