-
Product Name
POLE3 antibody
- Documents
-
Description
POLE3 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver cancer tissue. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, MCF7 cells. Observed molecular weight by Western-blot: 19 kDa
-
Tested applications
ELISA, WB, IHC, IP, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
Arsenic transactivated protein antibody; AsTP antibody; CHARAC17 antibody; CHRAC 17 antibody; CHRAC17 antibody; DNA polymerase II subunit 3 antibody; HuCHRAC17 antibody; p17 antibody; POLE3 antibody; YBL1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of POLE3 recombinant protein (Accession Number: NM_017443). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
-
Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114001(POLE3 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114001(POLE3 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114001(POLE3 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-POLE3 (IP:Catalog No:114001, 4ug; Detection:Catalog No:114001 1:1000) with HepG2 cells lysate 1800ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:114001(POLE3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
POLE3 represents polymerase (DNA directed), epsilon 3 (p17 subunit)
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"