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Product Name
POLD3 antibody
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Description
POLD3 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human cervical cancer tissue, human breast cancer tissue. Positive IP detected in MCF-7 cells. Positive WB detected in MCF-7 cells, mouse spleen tissue. Observed molecular weight by Western-blot: 51-70 kDa
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Tested applications
ELISA, WB, IP, IF, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DNA polymerase delta subunit 3 antibody; KIAA0039 antibody; P66 antibody; P68 antibody; POLD3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of POLD3 recombinant protein (Accession Number: BC108908). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113999(POLD3 Antibody) at dilution of 1:300
IP Result of anti-POLD3 (IP:Catalog No:113999, 4ug; Detection:Catalog No:113999 1:300) with MCF-7 cells lysate 2560ug.
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:113999(POLD3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:113999(POLD3 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells using Catalog No:113999(POLD3 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
POLD3 represents polymerase (DNA-directed), delta 3, accessory subunit
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"