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Product Name
POFUT1 antibody
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Description
POFUT1 Rabbit Polyclonal antibody. Positive IF detected in A431 cells. Positive IHC detected in human liver cancer tissue, mouse liver tissue. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, human brain tissue, human heart tissue, human kidney tissue, human liver tissue. Observed molecular weight by Western-blot: 44kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FUT12 antibody; KIAA0180 antibody; O Fuc T antibody; O FucT 1 antibody; O FUT antibody; Peptide O fucosyltransferase 1 antibody; POFUT1 antibody; protein O fucosyltransferase 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of POFUT1 recombinant protein (Accession Number: NM_172236). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113993(POFUT1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:113993(POFUT1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:113993(POFUT1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A431 cells, using POFUT1 antibody Catalog No:113993 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-POFUT1 (IP:Catalog No:113993, 4ug; Detection:Catalog No:113993 1:500) with HepG2 cells lysate 2400ug.
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Background
POFUT1 (protein O-fucosyltransferase 1), also known as GDP-fucose protein O-fucosyltransferase, is an enzyme that catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains and plays a crucial role in Notch signaling.
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References
- Annani-Akollor ME, Wang S, Fan J, Liu L, Padhiar AA, Zhang J. Downregulated protein O-fucosyl transferase 1 (Pofut1) expression exerts antiproliferative and antiadhesive effects on hepatocytes by inhibiting Notch signalling. Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie. 68(6):785-90. 2014.
- Liu S, Wang J, Qin HM. LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface. Cell death & disease. 5:e1396. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"