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Product Name
PNRC2 antibody
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Description
PNRC2 Rabbit Polyclonal antibody. Positive IP detected in mouse lung tissue. Positive WB detected in mouse lung tissue. Positive IHC detected in human gliomas tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 25kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ20312 antibody; PNRC2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PNRC2 recombinant protein (Accession Number: NM_017761). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:113986(PNRC2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113986(PNRC2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113986(PNRC2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using PNRC2 antibody Catalog No: at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-PNRC2 (IP:Catalog No:113986, 4ug; Detection:Catalog No:113986 1:300) with mouse lung tissue lysate 4000ug.
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Background
PNRC2 belongs to the PNRC family and PNRC2 subfamily. It is Involved in nonsense-mediated mRNA decay (NMD) by acting as a bridge between the mRNA decapping complex and the NMD machinery. It is required for UPF1/RENT1 localization to the P-body. PNRC2 also acts as a nuclear receptor coactivator. It may play a role in controlling the energy balance between energy storage and energy expenditure. (PMID:19150429) PNRC2 functions as a coactivator of several nuclear receptors, including ERRa, and participates in the activation of aromatase in conjunction with ERRa. Growth factor signaling promotes PELP1 recruitment to the aromatase chromatin and its formation of a complex with ERRa and PNRC2.(PMID:18079323)
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References
- Rajhans R, Nair HB, Nair SS. Modulation of in situ estrogen synthesis by proline-, glutamic acid-, and leucine-rich protein-1: potential estrogen receptor autocrine signaling loop in breast cancer cells. Molecular endocrinology (Baltimore, Md.). 22(3):649-64. 2008.
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