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Product Name
PNPT1 antibody
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Description
PNPT1 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue, human brain tissue, human kidney tissue, human lung tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
3 5 RNA exonuclease OLD35 antibody; DKFZp762K1914 antibody; old 35 antibody; OLD35 antibody; PNPASE antibody; PNPase 1 antibody; PNPase old 35 antibody; PNPT1 antibody; Polynucleotide phosphorylase 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PNPT1 recombinant protein (Accession Number: NM_033109). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113984(PNPT1 antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113984(PNPT1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113984(PNPT1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-PNPT1 (IP:Catalog No:113984, 4ug; Detection:Catalog No:113984 1:1000) with mouse brain tissue lysate 4000ug.
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Background
PNPT1 (polynucleotide phosphorylase1; also known as PNPASE) localizes in the mitochondrial intermembrane and regulates RNA import into mitochondria (20691904). PNPT1 is also involved in mRNA degradation. As a type I IFN-inducible gene, PNPT1 plays an essential role in mediating IFN-mediated growth inhibition and inflammatory processes (17804700). Recently mutation in PNPT1 has been reported to cause hereditary hearing loss (23084290). This antibody detected the endogenous PNPT1 around 80 kDa in mouse brain.
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References
- von Ameln S, Wang G, Boulouiz R. A mutation in PNPT1, encoding mitochondrial-RNA-import protein PNPase, causes hereditary hearing loss. American journal of human genetics. 91(5):919-27. 2012.
- Pangršič T, Gabrielaitis M, Michanski S. EF-hand protein Ca2+ buffers regulate Ca2+ influx and exocytosis in sensory hair cells. Proceedings of the National Academy of Sciences of the United States of America. 112(9):E1028-37. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"