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Product Name
PMPCA Polyclonal Antibody
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Description
Polyclonal antibody to PMPCA
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
PMPCA antibody; Alpha-MPP antibody; INPP5E antibody; P-55 antibody; SCAR2 antibody; mitochondrial-processing peptidase subunit alpha antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 456-525 of human PMPCA (NP_055975.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - PMPCA Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PMPCA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which is required for maturation of the majority of mitochondrial precursor proteins. Most MPP cleavage sites follow an arginine at position -2 (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"