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Product Name
PMM2 antibody
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Description
PMM2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue. Positive WB detected in HepG2 cells, HeLa cells. Positive IP detected in mouse lung tissue. Observed molecular weight by Western-blot: 28-30 kDa
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Tested applications
ELISA, IHC, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDG1 antibody; CDG1a antibody; CDGS antibody; phosphomannomutase 2 antibody; PMM 2 antibody; PMM2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PMM2 recombinant protein (Accession Number: NM_000303). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
WB results of Catalog No:114026(PMM2 antibody) on several lysates.
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114026(PMM2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:114026(PMM2 antibody) at dilution of 1:100 (under 25x lens)
IP Result of anti-PMM2 (IP:Catalog No:114026, 4ug; Detection:Catalog No:114026 1:1000) with mouse lung tissue lysate 3600ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:114026(PMM2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PMM2(Phosphomannomutase 2) belongs to the eukaryotic PMM family. It is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. PMM2 catalyzes the second step in the conversion of fructose-6P to GDP-mannose.
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References
- Wegrzynowicz M, Holt HK, Friedman DB, Bowman AB. Changes in the striatal proteome of YAC128Q mice exhibit gene-environment interactions between mutant huntingtin and manganese. Journal of proteome research. 11(2):1118-32. 2012.
- Himmelreich N, Kaufmann LT, Steinbeisser H, Körner C, Thiel C. Lack of phosphomannomutase 2 affects Xenopus laevis morphogenesis and the non-canonical Wnt5a/Ror2 signalling. Journal of inherited metabolic disease. 38(6):1137-46. 2015.
- Andreotti G, Monti MC, Citro V, Cubellis MV. Heterodimerization of Two Pathological Mutants Enhances the Activity of Human Phosphomannomutase2. PloS one. 10(10):e0139882. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"