Product Name

PLOD3 antibody

Documents

Description

PLOD3 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas cancer tissue, human kidney tissue, human pancreas tissue. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, A549 cells, HeLa cells, human placenta tissue, mouse placenta tissue, PC-3 cells. Observed molecular weight by Western-blot: 80-85 kDa

Tested applications

ELISA, WB, IHC, IP, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

LH3 antibody; Lysyl hydroxylase 3 antibody; PLOD3 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of PLOD3 recombinant protein (Accession Number: NM_001084). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IP: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:114006(PLOD3 antibody) at dilution of 1:800

IP Result of anti-PLOD3 (IP:Catalog No:114006, 3ug; Detection:Catalog No:114006 1:500) with HepG2 cells lysate 6000ug.

Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:114006(PLOD3 antibody) at dilution of 1:50 (under 10x lens)

Immunofluorescent analysis of HepG2 cells using Catalog No:114006(PLOD3 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Background

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

References

Salo AM, Cox H, Farndon P. A connective tissue disorder caused by mutations of the lysyl hydroxylase 3 gene. American journal of human genetics. 83(4):495-503. 2008.
Wang C, Kovanen V, Raudasoja P, Eskelinen S, Pospiech H, Myllylä R. The glycosyltransferase activities of lysyl hydroxylase 3 (LH3) in the extracellular space are important for cell growth and viability. Journal of cellular and molecular medicine. 13(3):508-21. 2009.
Risteli M, Ruotsalainen H, Salo AM. Reduction of lysyl hydroxylase 3 causes deleterious changes in the deposition and organization of extracellular matrix. The Journal of biological chemistry. 284(41):28204-11. 2009.
Bunt S, Denholm B, Skaer H. Characterisation of the Drosophila procollagen lysyl hydroxylase, dPlod. Gene expression patterns : GEP. 11(1-2):72-8. 2011.
Wang C, Ristiluoma MM, Salo AM, Eskelinen S, Myllylä R. Lysyl hydroxylase 3 is secreted from cells by two pathways. Journal of cellular physiology. 227(2):668-75. 2012.
Lai Y, Song M, Hakala K, Weintraub ST, Shiio Y. Proteomic dissection of the von Hippel-Lindau (VHL) interactome. Journal of proteome research. 10(11):5175-82. 2011.
Witsch TJ, Turowski P, Sakkas E. Deregulation of the lysyl hydroxylase matrix cross-linking system in experimental and clinical bronchopulmonary dysplasia. American journal of physiology. Lung cellular and molecular physiology. 306(3):L246-59. 2014.
Cabral WA, Perdivara I, Weis M. Abnormal type I collagen post-translational modification and crosslinking in a cyclophilin B KO mouse model of recessive osteogenesis imperfecta. PLoS genetics. 10(6):e1004465. 2014.

PLOD3 antibody

Product Information

Related Products / Services