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  • PLOD3 antibody
  • PLOD3 antibody
  • PLOD3 antibody
  • PLOD3 antibody
  • PLOD3 antibody

PLOD3 antibody

Cat.#: 107477

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Special Price 239.0 USD

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Product Information

  • Product Name
    PLOD3 antibody
  • Documents
  • Description
    PLOD3 Mouse Monoclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in Human placenta, PC-3 cells. Positive IF detected in HeLa cells. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 80-85 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    LH3 antibody; Lysyl hydroxylase 3 antibody; PLOD3 antibody
  • Isotype
    Mouse IgG2a
  • Preparation
    This antibody was obtained by immunization of PLOD3 recombinant protein (Accession Number: BC011674). Purification method: Caprylic Acid/Ammonium Sulfate Precipitation.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:107477(PLOD3 antibody) at dilution of 1:300

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:107477(PLOD3 antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:107477(PLOD3 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:107477(PLOD3 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:107477(PLOD3 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:107477(PLOD3 antibody) at dilution of 1:100 (under 40x lens)

    IP Result of anti-PLOD3 (IP:Catalog No:107477, 5ug; Detection:Catalog No:107477 1:500) with HeLa cells lysate 1400ug.

    IP Result of anti-PLOD3 (IP:Catalog No:107477, 5ug; Detection:Catalog No:107477 1:500) with HeLa cells lysate 1400ug.

    Immunofluorescent analysis of HeLa cells using Catalog No:107477(PLOD3 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG

    Immunofluorescent analysis of HeLa cells using Catalog No:107477(PLOD3 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG

  • Background
    PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.
  • References
    • Watt SA, Dayal JH, Wright S. Lysyl Hydroxylase 3 Localizes to Epidermal Basement Membrane and Is Reduced in Patients with Recessive Dystrophic Epidermolysis Bullosa. PloS one. 10(9):e0137639. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"