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Product Name
PLOD3 antibody
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Description
PLOD3 Mouse Monoclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in Human placenta, PC-3 cells. Positive IF detected in HeLa cells. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 80-85 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
LH3 antibody; Lysyl hydroxylase 3 antibody; PLOD3 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of PLOD3 recombinant protein (Accession Number: NM_001084). Purification method: Caprylic Acid/Ammonium Sulfate Precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:107477(PLOD3 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:107477(PLOD3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:107477(PLOD3 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-PLOD3 (IP:Catalog No:107477, 5ug; Detection:Catalog No:107477 1:500) with HeLa cells lysate 1400ug.
Immunofluorescent analysis of HeLa cells using Catalog No:107477(PLOD3 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG
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Background
PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.
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References
- Watt SA, Dayal JH, Wright S. Lysyl Hydroxylase 3 Localizes to Epidermal Basement Membrane and Is Reduced in Patients with Recessive Dystrophic Epidermolysis Bullosa. PloS one. 10(9):e0137639. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"