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PLOD2-Specific antibody

Cat.#: 114005

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Product Information

  • Product Name
    PLOD2-Specific antibody
  • Documents
  • Description
    PLOD2-Specific Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IF detected in Hela cells. Positive IHC detected in human liver cancer tissue, human pancreas tissue, human skeletal muscle tissue. Positive WB detected in DU 145 cells, HT-1080 cells, mouse liver tissue, mouse pancreas tissue, mouse testis tissue. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 85 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    LH2 antibody; Lysyl hydroxylase 2 antibody; PLOD2-Specific antibody; TLH antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PLOD2-Specific recombinant protein (Accession Number: NM_182943). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    DU 145 cells were subjected to SDS PAGE followed by western blot with Catalog No:114005(PLOD2 antibody) at dilution of 1:300

    DU 145 cells were subjected to SDS PAGE followed by western blot with Catalog No:114005(PLOD2 antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114005(PLOD2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114005(PLOD2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114005(PLOD2 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114005(PLOD2 antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of Hela cells, using PLOD2 antibody Catalog No:114005 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of Hela cells, using PLOD2 antibody Catalog No:114005 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-PLOD2 (IP:Catalog No:114005, 4ug; Detection:Catalog No:114005 1:500) with mouse testis tissue lysate 5000ug.

    IP Result of anti-PLOD2 (IP:Catalog No:114005, 4ug; Detection:Catalog No:114005 1:500) with mouse testis tissue lysate 5000ug.

    1X10^6 HeLa cells were stained with 0.2ug PLOD2 antibody (Catalog No:114005, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 HeLa cells were stained with 0.2ug PLOD2 antibody (Catalog No:114005, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.
  • References
    • Noda T, Yamamoto H, Takemasa I. PLOD2 induced under hypoxia is a novel prognostic factor for hepatocellular carcinoma after curative resection. Liver international : official journal of the International Association for the Study of the Liver. 32(1):110-8. 2012.
    • Remst DF, Blaney Davidson EN, Vitters EL. Osteoarthritis-related fibrosis is associated with both elevated pyridinoline cross-link formation and lysyl hydroxylase 2b expression. Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society. 21(1):157-64. 2013.
    • Eisinger-Mathason TS, Zhang M, Qiu Q. Hypoxia-dependent modification of collagen networks promotes sarcoma metastasis. Cancer discovery. 3(10):1190-205. 2013.
    • Remst DF, Blaney Davidson EN, Vitters EL, Bank RA, van den Berg WB, van der Kraan PM. TGF-ß induces Lysyl hydroxylase 2b in human synovial osteoarthritic fibroblasts through ALK5 signaling. Cell and tissue research. 355(1):163-71. 2014.
    • there was a problem with your PMID, try again.
    • Remst DF, Blom AB, Vitters EL. Gene expression analysis of murine and human osteoarthritis synovium reveals elevation of transforming growth factor β-responsive genes in osteoarthritis-related fibrosis. Arthritis & rheumatology (Hoboken, N.J.). 66(3):647-56. 2014.
    • Chen Y, Terajima M, Yang Y. Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma. The Journal of clinical investigation. 125(3):1147-62. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"