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Product Name
PLOD1 Polyclonal Antibody
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Description
Polyclonal antibody to PLOD1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
PLOD1 antibody; EDS6 antibody; LH antibody; LH1 antibody; LLH antibody; PLOD antibody; procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 488-727 of human PLOD1 (NP_000293.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - PLOD1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PLOD1 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
Immunofluorescence - PLOD1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using PLOD1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (By similarity). Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (Probable).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"