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Product Name
PLK3 antibody
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Description
PLK3 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in Transfected HEK-293 cells. Observed molecular weight by Western-blot: 72 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CNK antibody; FGF inducible kinase antibody; FNK antibody; PLK 3 antibody; PLK3 antibody; Polo like kinase 3 antibody; PRK antibody; Proliferation related kinase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PLK3 recombinant protein (Accession Number: NM_004073). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113970(PLK3 antibody) at dilution of 1:10 (under 10x lens)
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113970(PLK3 Antibody) at dilution of 1:1000
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Background
PLK3(Polo-like kinase 3) is also named as CNK, FNK, PRK and belongs to the protein kinase superfamily. It is involved in the regulation of DNA damage checkpoint as well as in M-phase function. Plk3 physically interacts with p53 and phosphorylates this tumor suppressor protein on serine-20, suggesting that the role of Plk3 in cell cycle progression is mediated, at least in part, through direct regulation of p53.(PMID:12548019).
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References
- Fingas CD, Mertens JC, Razumilava N. Polo-like kinase 2 is a mediator of hedgehog survival signaling in cholangiocarcinoma. Hepatology (Baltimore, Md.). 58(4):1362-74. 2013.
- Singh L, Pushker N, Sen S, Singh MK, Chauhan FA, Kashyap S. Prognostic significance of polo-like kinases in retinoblastoma: correlation with patient outcome, clinical and histopathological parameters. Clinical & experimental ophthalmology. 43(6):550-7. 2015.
- López-Sánchez I, Sanz-García M, Lazo PA. Plk3 interacts with and specifically phosphorylates VRK1 in Ser342, a downstream target in a pathway that induces Golgi fragmentation. Molecular and cellular biology. 29(5):1189-201. 2009.
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