PLK2 Polyclonal Antibody

Cat.#: 165166

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Product Information

  • Product Name
    PLK2 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to PLK2
  • Tested applications
    WB, IHC
  • Species reactivity
    Human, Mouse
  • Alternative names
    PLK2 antibody; SNK antibody; hPlk2 antibody; hSNK antibody; polo like kinase 2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 322-671 of human PLK2 (NP_001239155.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:100 - 1:200
  • Validations

    Western blot - PLK2 Polyclonal Antibody

    Western blot - PLK2 Polyclonal Antibody

    Western blot analysis of extracts of MCF-7 cells, using PLK2 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 300s.

  • Background
    Tumor suppressor serine/threonine-protein kinase involved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"