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Product Name
PLEKHF1 antibody
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Description
PLEKHF1 Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive IF detected in HepG2 cells. Positive WB detected in human skeletal muscle tissue, human kidney tissue, mouse lung tissue, mouse skeletal muscle tissue, mouse spleen tissue, mouse thymus tissue. Observed molecular weight by Western-blot: 31kd
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Apoptosis inducing protein antibody; APPD antibody; LAPF antibody; Phafin 1 antibody; PHAFIN1 antibody; PLEKHF1 antibody; ZFYVE15 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PLEKHF1 recombinant protein (Accession Number: NM_024310). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:113961(PLEKHF1 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human brain slide using Catalog No:113961(PLEKHF1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human brain slide using Catalog No:113961(PLEKHF1 Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells, using PLEKHF1 antibody Catalog No:113961 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
PLEKHF1, also named as APPD, LAPF, and ZFYVE15, is a 279 amino acid protein, which contains one PH domain and one FYVE-type zinc finger. PLEKHF1 translocates to lysosome during apoptosis, localizing in the nucleus and cytoplsam. PLEKHF1 is highly expressed in heart and skeletal muscle and weakly expressed in brain, thymus, spleen, kidney, liver, small intestine, placenta and lung. PLEKHF1 may induce apoptosis through the lysosomal-mitochondrial pathway. PLEKHF1 modulates the protein content of the plasma membrane and was involved in different endocytosis events.
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References
- Natrajan R, Mackay A, Wilkerson PM. Functional characterization of the 19q12 amplicon in grade III breast cancers. Breast cancer research : BCR. 14(2):R53. 2012.
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