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PLD1 antibody

Cat.#: 113951

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Product Information

  • Product Name
    PLD1 antibody
  • Documents
  • Description
    PLD1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 120 kDa
  • Tested applications
    ELISA, WB, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Choline phosphatase 1 antibody; hPLD1 antibody; Phospholipase D1 antibody; PLD 1 antibody; PLD1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PLD1 recombinant protein (Accession Number: XM_005247533). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113951(PLD1 antibody) at dilution of 1:500

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113951(PLD1 antibody) at dilution of 1:500

    IP Result of anti-PLD1 (IP:Catalog No:113951, 4ug; Detection:Catalog No:113951 1:500) with HeLa cells lysate 1200ug.

    IP Result of anti-PLD1 (IP:Catalog No:113951, 4ug; Detection:Catalog No:113951 1:500) with HeLa cells lysate 1200ug.

    Immunofluorescent analysis of HeLa cells using Catalog No:113951(PLD1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HeLa cells using Catalog No:113951(PLD1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    PLD1(Phospholipase D1) belongs to the phospholipase D family. It is implicated in the release process of a variety of hormones, most likely at a late stage of exocytosis through the production of lipids that are essential for the fusion process and seems to be also essential for fast neurotransmitter release. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes and autophagosomes.
  • References
    • Yoon MS, Du G, Backer JM, Frohman MA, Chen J. Class III PI-3-kinase activates phospholipase D in an amino acid-sensing mTORC1 pathway. The Journal of cell biology. 195(3):435-47. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"