Mouse PDGF-C (Fc Tag) recombinant protein

Mouse PDGF-C (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function.

Platelet-derived growth factor C

SCDGF; FALLOTEIN; AI647969; 1110064L01RIK; PDGFC; PDGF-C

Q8CI19

Pdgfc; Scdgf

Human Cells

A DNA sequence encoding the receptor-binding form of mouse PDGFC (Q8CI19) (Val 235-Gly 345) was fused with the Fc region of human IgG1 at the N-terminus.

Mouse

The recombinant mouse PDGFC/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 372 amino acids and has a predicted molecular mass of 40.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmPDGFC/Fc monomer is approximately 46 kDa.

> 80 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized mouse PDGF-C (Cat:501764) at 10 μg/mL (100 μl/well) can bind  human PDGFRA (Cat:503138), The EC₅₀ of human PDGFRA (Cat:503138) is 0.71 μg/mL.
3. Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is typically 0.5-2 μg/mL.