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Product Name
PLA2G2D Polyclonal Antibody
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Description
Polyclonal antibody to PLA2G2D
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Rat
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Alternative names
PLA2G2D antibody; PLA2IID antibody; SPLASH antibody; sPLA2-IID antibody; sPLA2S antibody; phospholipase A2 group IID antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-145 of human PLA2G2D (NP_036532.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100 -
Validations
Western blot - PLA2G2D Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PLA2G2D antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - PLA2G2D Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using PLA2G2D antibody . Blue: DAPI for nuclear staining.
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Background
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2-linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"