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PKM2-specific antibody

Cat.#: 107474

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Product Information

  • Product Name
    PKM2-specific antibody
  • Documents
  • Description
    PKM2-specific Mouse Monoclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Positive WB detected in multi-cells/tissue, human brain tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 58 kDa
  • Tested applications
    ELISA, IF, WB, FC, IP, IHC
  • Species reactivity
    Human,Mouse, Rat; other species not tested.
  • Alternative names
    CTHBP antibody; OIP 3 antibody; OIP3 antibody; Opa interacting protein 3 antibody; p58 antibody; PK2 antibody; PK3 antibody; PKM antibody; PKM1 antibody; PKM2 antibody; PKM2 pyruvate kinase antibody; muscle antibody; Pyruvate kinase 2/3 antibody; Pyruvate kinase isozymes M1/M2 antibody; Pyruvate kinase muscle isozyme antibody; TCB antibody; THBP1 antibody; Tumor M2 PK antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_002654). Purification method: Protein G purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:1000

    IHC: 1:50-1:500

    IF: 1:20-1:200

  • Validations

    Immunofluorescent analysis of HepG2 cells using Catalog No:107474(PK-M2-specific Antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red).

    Immunofluorescent analysis of HepG2 cells using Catalog No:107474(PK-M2-specific Antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red).

    Western blot analysis of PK-M2-specific in various tissues and cell lines using Proteintech antibody Catalog No:107474 at dilution of 1:1000

    Western blot analysis of PK-M2-specific in various tissues and cell lines using Proteintech antibody Catalog No:107474 at dilution of 1:1000

    IP Result of anti-PK-M2-specific (IP:Catalog No:107474, 5ug; Detection:Catalog No:107474 1:300) with HeLa cells lysate 2800ug.

    IP Result of anti-PK-M2-specific (IP:Catalog No:107474, 5ug; Detection:Catalog No:107474 1:300) with HeLa cells lysate 2800ug.

    1X10^6 HepG2 cells were stained with .2ug PK-M2-specific antibody (Catalog No:107474, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with .2ug PK-M2-specific antibody (Catalog No:107474, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

  • Background
    PKM2, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM2 plays a general role in caspase independent cell death of tumor cells. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells.The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823) PKM2, Pyruvate kinase isozymes M1/M2, has 2 isoforms .The immunogen of this antibody is M2 isoform, also called PKM2.
  • References
    • Lu W, Zhang Y, Zhou L. miR-122 inhibits cancer cell malignancy by targeting PKM2 in gallbladder carcinoma. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"