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PKM2-specific antibody

Cat.#: 106995

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Product Information

  • Product Name
    PKM2-specific antibody
  • Documents
  • Description
    PKM2-specific Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, 293 cell, C6 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, mouse brain tissue, mouse muscle/liver tissue, mouse skeletal muscle tissue, Recombinant protein. Positive IP detected in MCF-7 cells. Positive IHC detected in human lung cancer tissue, human colon cancer tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 58 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CTHBP antibody; OIP 3 antibody; OIP3 antibody; Opa interacting protein 3 antibody; p58 antibody; PK2 antibody; PK3 antibody; PKM antibody; PKM2 antibody; PK-M2 antibody; PKM2 pyruvate kinase antibody; muscle antibody; Pyruvate kinase 2/3 antibody; Pyruvate kinase isozymes M1/M2 antibody; Pyruvate kinase muscle isozyme antibody; TCB antibody; THBP1 antibody; Tumor M2 PK antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_002654). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:1000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    WB results of Catalog No:113861(PKM2 antibody) at dilution of 1:1500.

    WB results of Catalog No:113861(PKM2 antibody) at dilution of 1:1500.

    WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control.)

    WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control.)

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113861(PK-M2-specific antibody) at dilution of 1:1500

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113861(PK-M2-specific antibody) at dilution of 1:1500

    IP Result of anti-PK-M2-specific (IP:Catalog No:113861, 4ug; Detection:Catalog No:113861 1:300) with MCF-7 cells lysate 3250ug.

    IP Result of anti-PK-M2-specific (IP:Catalog No:113861, 4ug; Detection:Catalog No:113861 1:300) with MCF-7 cells lysate 3250ug.

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113861(PK-M2-specific antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113861(PK-M2-specific antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113861(PK-M2-specific antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113861(PK-M2-specific antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of MCF-7 cells, using PKM2 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of MCF-7 cells, using PKM2 antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Background
    PKM2, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM2 plays a general role in caspase independent cell death of tumor cells. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells.The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823) PKM2, Pyruvate kinase isozymes M1/M2, has 2 isoforms .The immunogen of this antibody is M2 isoform, also called PKM2. This antibody is specific to PKM2 isoform.
  • References
    • Yuan Y, Guo-Qing P, Yan T, Hong-Lin Y, Gong-Hua H, Cai-Gao Z. A study of PKM2, PFK-1, and ANT1 expressions in cervical biopsy tissues in China. Medical oncology (Northwood, London, England). 29(4):2904-10. 2012.
    • Nakatsu D, Horiuchi Y, Kano F. L-cysteine reversibly inhibits glucose-induced biphasic insulin secretion and ATP production by inactivating PKM2. Proceedings of the National Academy of Sciences of the United States of America. 112(10):E1067-76. 2015.
    • Cautain B, de Pedro N, Schulz C. Identification of the Lipodepsipeptide MDN-0066, a Novel Inhibitor of VHL/HIF Pathway Produced by a New Pseudomonas Species. PloS one. 10(5):e0125221. 2015.
    • Konno M, Koseki J, Kawamoto K. Embryonic MicroRNA-369 Controls Metabolic Splicing Factors and Urges Cellular Reprograming. PloS one. 10(7):e0132789. 2015.
    • Martinez-Outschoorn UE, Balliet RM, Rivadeneira DB. Oxidative stress in cancer associated fibroblasts drives tumor-stroma co-evolution: A new paradigm for understanding tumor metabolism, the field effect and genomic instability in cancer cells. Cell cycle (Georgetown, Tex.). 9(16):3256-76. 2010.
    • Chiavarina B, Whitaker-Menezes D, Martinez-Outschoorn UE. Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth. Cancer biology & therapy. 12(12):1101-13. 2011.
    • Bonuccelli G, Whitaker-Menezes D, Castello-Cros R. The reverse Warburg effect: glycolysis inhibitors prevent the tumor promoting effects of caveolin-1 deficient cancer associated fibroblasts. Cell cycle (Georgetown, Tex.). 9(10):1960-71. 2010.
    • Migneco G, Whitaker-Menezes D, Chiavarina B. Glycolytic cancer associated fibroblasts promote breast cancer tumor growth, without a measurable increase in angiogenesis: evidence for stromal-epithelial metabolic coupling. Cell cycle (Georgetown, Tex.). 9(12):2412-22. 2010.

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