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Product Name
PKC epsilon antibody
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Description
PKC epsilon Rabbit Polyclonal antibody. Positive WB detected in SH-SY5Y cells, mouse brain tissue. Positive IP detected in SH-SY5Y cells. Positive IHC detected in human thyroid cancer tissue. Observed molecular weight by Western-blot: 84 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PKC epsilon antibody; PKCE antibody; PRKCE antibody; Protein kinase C epsilon type antibody; protein kinase C antibody; epsilon antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PKC epsilon recombinant protein (Accession Number: NM_005400). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:113850(PRKCE antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human thyroid cancer using Catalog No:113850(PRKCE antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human thyroid cancer using Catalog No:113850(PRKCE antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-PRKCE (IP:Catalog No:113850, 4ug; Detection:Catalog No:113850 1:800) with SH-SY5Y cells lysate 1600ug.
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Background
PRKCE represents protein kinase C, epsilon
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References
- Zhao W, Wang P, Ma J. MiR-34a regulates blood-tumor barrier function by targeting protein kinase Cε. Molecular biology of the cell. 26(10):1786-96. 2015.
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